Maize Type II callus tissue was used as the plant material for genetic transformation via electroporation. Plasmid DNA containing a selectable marker gene (either neomycin phosphotransferase (npt-II) or phosphinothricin acetyl transferase (bar)), and a screenable marker gene (gus A) was incubated wi
Transformation of maize embryogenic calli mediated by ultrasonication and regeneration of fertile transgenic plants
โ Scribed by Hong Zhang; Guoying Wang; Youju Xie; Jingrui Dai; Ning Xu; Nanming Zhao; Taiyuan Li; Yingchuan Tian; Liya Qiao; Keqiang Mang
- Publisher
- Science in China Press (SCP)
- Year
- 1997
- Tongue
- English
- Weight
- 458 KB
- Volume
- 40
- Category
- Article
- ISSN
- 1674-7305
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Regeneration of pepino (Solanum muricatum Ait.) shoots was achieved both by organogenesis and by embryogenesis. Shoots derived via organogenesis were easily rooted and most regenerated plants appeared phenotypically normal. Transgenic plants were obtained using the binary vector pKIWI110 in the avir
Fertile plants have been obtained from maize (Zea mays L.) embryogenic suspension culture protoplasts. Friable, embryogenic callus initiated from an immature embryo from a cross involving the genotypes A188, B73, and Black Mexican sweetcorn was used to establish a rapidly growing embryogenic suspens