𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Agrobacterium-mediated transformation of pepino and regeneration of transgenic plants

✍ Scribed by Roos G. Atkinson; Richard C. Gardner

Publisher
Springer
Year
1991
Tongue
English
Weight
880 KB
Volume
10
Category
Article
ISSN
0721-7714

No coin nor oath required. For personal study only.

✦ Synopsis

Regeneration of pepino (Solanum muricatum Ait.) shoots was achieved both by organogenesis and by embryogenesis. Shoots derived via organogenesis were easily rooted and most regenerated plants appeared phenotypically normal. Transgenic plants were obtained using the binary vector pKIWI110 in the avirulent Agrobacterium tumefaciens strain LBA4404. Optimization of transformation protocols was rapidly achieved by monitoring early expression of the GUS (Ξ²-D-glucuronidase) reporter gene carried on pKIWI110. Transgenic plants expressed GUS and selectable marker genes for kanamycin resistance and chlorsulfuron resistance. PCR (polymerase chain reaction) and Southern analysis provided molecular evidence for transformation.

πŸ“œ SIMILAR VOLUMES

Agrobacterium-mediated transformation an
Agrobacterium-mediated transformation and plant regeneration of buckwheat (Fagopyrum esculentumMoench.)
✍ Jovanka MiljuΕ‘-DjukiΔ‡; Mirjana NeΕ‘koviΔ‡; Slavica Ninkovi#x0107;; Radomir Crkvenj πŸ“‚ Article πŸ“… 1992 πŸ› Springer 🌐 English βš– 29 KB

On page 102 of the above article, a line of text was inadvertently omitted from the left-hand column. The bottom six lines, following "was supplemented with", should read as follows: -5mgl-1 24-D and 0.1mgl-1 of kinetin to induce cell division, or -2.2 mg 1 -~ BAP and 0.17 mg 1 -~ IAA to induce org

Agrobacterium tumefaciensmediated transf
Agrobacterium tumefaciensmediated transformation and regeneration of muskmelon plants
✍ Guowei Fang; Rebecca Grumet πŸ“‚ Article πŸ“… 1990 πŸ› Springer 🌐 English βš– 625 KB

Transgenic muskmelon (Cucumis melo L.) plants were produced efficiently by inoculating cotyledon explants with Agrobacterium tumefaciens strain LBA4404 bearing a Ti plasmid with the NPT II gene for kanaymcin resistance. After co-cultivation for three days, expiants were transferred to melon regenera

Agrobacterium-mediated transformation of
Agrobacterium-mediated transformation of peanut (Arachis hypogaeaL.) embryo axes and the development of transgenic plants
✍ A. H. McKently; G. A. Moore; H. Doostdar; R. P. Niedz πŸ“‚ Article πŸ“… 1995 πŸ› Springer 🌐 English βš– 831 KB

Transgenic peanut (Arachis hypogaea L.) plants have been produced using an Agrobacterium-mediated transformation system. Zygotic embryo axes from mature seed were cocultured with Agrobacterium tumefaciens strain EHA101 harboring a binary vector that contained the genes for the scorable marker B-gluc