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Agrobacterium tumefaciensmediated transformation and regeneration of muskmelon plants

✍ Scribed by Guowei Fang; Rebecca Grumet

Publisher: Springer
Year: 1990
Tongue: English
Weight: 625 KB
Volume: 9
Category: Article
ISSN: 0721-7714
DOI: 10.1007/bf00232095

No coin nor oath required. For personal study only.

✦ Synopsis

Transgenic muskmelon (Cucumis melo L.) plants were produced efficiently by inoculating cotyledon explants with Agrobacterium tumefaciens strain LBA4404 bearing a Ti plasmid with the NPT II gene for kanaymcin resistance. After co-cultivation for three days, expiants were transferred to melon regeneration medium with kanamycin to select for transformed tissue. Shoot regeneration occurred within 3-5 weeks; excised shoots were rooted on medium containing kanamycin before transferring to soil. Morphologically normal plants were produced in three months. Southern blot analysis confirmed that ca. 85% of the regenerated plants contained the NPT gene. Dot blot analysis and leaf callus assay of progeny of transgenic plants verified transmission of the introduced gene(s) to the next generation. Factors affecting transformation efficiency are discussed.

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