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Plant regeneration from cotyledon protoplasts of Xinjiang muskmelon

โœ Scribed by Renjing Li; Yongru Sun; Liming Zhang; Xianghui Li

Publisher
Springer
Year
1990
Tongue
English
Weight
957 KB
Volume
9
Category
Article
ISSN
0721-7714

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โœฆ Synopsis

Cotyledon protoplasts were isolated from seedlings of Xinjiang muskelon (Cucumis melo var.saccharinus) grown under sterile conditions and cultured in modified Miller medium. High frequency division of the protoplast-derived cells was observed. Agarose bead culture with B6S3 tobacco crown gall nurse cells was found most suitable for the cotyledon protoplasts when compared with other culture methods. Intact plants were regenerated from the protocalli by a two-step culture procedure with liquid and then solid media.

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