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Transformation in Bacillus subtilis

โœ Scribed by Arwert, F. ;Venema, G.

Publisher
Springer
Year
1973
Tongue
English
Weight
787 KB
Volume
123
Category
Article
ISSN
0026-8925

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Two HindIII generated DNA fragments of 3.0 and 2.3 Kb derived from rRNA genes of B. subtilis were cloned in E. coli with pBR322. The 3.0 Kb fragment could be subcloned in B. subtilis using pC194. However, only the multimeric, but not the monomeric derivatives of this hybrid plasmid were active in tr