๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Stimulation of Bacillus subtilis transformation by spermidine

โœ Scribed by Clark, Phillip O. ;Leach, Franklin R.

Publisher
Springer
Year
1980
Tongue
English
Weight
439 KB
Volume
178
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

๐Ÿ“œ SIMILAR VOLUMES

High frequency transformation of Bacillu
High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA
โœ Chang, Shing ;Cohen, Stanley N. ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 449 KB

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra

Restriction of plasmid-mediated transfor
Restriction of plasmid-mediated transformation in Bacillus subtilis 168
โœ Tanaka, Teruo ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 239 KB

When plasmids carrying leucine genes of Bacillus subtilis 168 were isolated from a restriction and modification deficient (r-m-) strain and used for transformation of a restricting strain B. subtilis 168 leu recE4, the number of transformants was greatly reduced. Transformation of rec+ strain (trans

Fate of plasmid DNA in transformation of
Fate of plasmid DNA in transformation of Bacillus subtilis protoplasts
โœ de Vos, Willem M. ;Venema, Gerard ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 603 KB

Polyethylene glycol-treated protoplasts of B. subtilis can be transformed by plasmid DNA at very high frequencies (Chang and Cohen 1979). From analysis of plasmid mediated transformation of transformation-deficient mutants it appeared that mutants, reduced in the transformation by plasmid DNA in the