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Transformation in Bacillus subtilis using excreted DNA

โœ Scribed by Streips, Uldis N. ;Young, Frank E.


Publisher
Springer
Year
1974
Tongue
English
Weight
540 KB
Volume
133
Category
Article
ISSN
0026-8925

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Transformation in Bacillus subtilis
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Plasmid transformation in Bacillus subti
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Two HindIII generated DNA fragments of 3.0 and 2.3 Kb derived from rRNA genes of B. subtilis were cloned in E. coli with pBR322. The 3.0 Kb fragment could be subcloned in B. subtilis using pC194. However, only the multimeric, but not the monomeric derivatives of this hybrid plasmid were active in tr

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A series of hybrid plasmids consisting of pC194 or pUBll2 and B. subtilis DNA were constructed. In contrast to plasmid pC 194, purified monomeric forms of such plasmids were active in transformation, provided the recipient cells were recombination proficient. Similarly the monomers of pC194 derived

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Following UV irradiation of Bacillus subtilis there is a coordinate induction of: 1) a new protein, 2) a W-reactivation system, 3) a DNA modification system, and 4) prophages. These functions are induced following UV irradiation of repair proficient bacteria and mutants deficient in excision repair