Transfer of proteins from acrylamide gels to nitrocellulose paper after silver stain detection
โ Scribed by Therasa J. Nalty; Lynn C. Yeoman
- Book ID
- 118900959
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 500 KB
- Volume
- 107
- Category
- Article
- ISSN
- 0022-1759
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๐ SIMILAR VOLUMES
A rapid picture of the extent of protein transfer from one-or two-dimensional polyacrylamide gels onto nitrocellulose membranes prior to immunodetection can be obtained by a rapid and reversible Amido Black staining procedure.
A rapid and highly sensitive silver stain and color stain were developed for visualizing proteins. The procedure is simple and the bands were clear. This silver stain detects 100 pg quantities of proteins. In order to stain quickly, sensitively, and sharply a protein matrix in a gel, the repeated sh
A method describing the rapid and effective transfer of integral membrane protein from isoelectric focusing gels to nitrocellulose is described. Initial experiments were carried out with detergentsolubilized extracts of human erythrocyte membrane proteins. The effectiveness of the transfer was demon