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Transcriptome analysis of barley anthers: effect of mannitol treatment on microspore embryogenesis

✍ Scribed by María Muñoz-Amatriaín; Jan T Svensson; Ana-María Castillo; Luís Cistué; Timothy J Close; María-Pilar Vallés

Book ID
110903026
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
195 KB
Volume
127
Category
Article
ISSN
0031-9317

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A period of four days preincubation at 25 °C on a medium containing mannitol was found to be superior to those pretreatments requiring incubation at 4 °C. In addition, the yield of green plants was improved by orienting anthers 'flat' on the medium during mannitol preincubation, and reducing the num

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The effect of anther orientation on culture response has been examined in a range of barley cultivars of agronomic importance. Anther orientation did not significantly affect the percentage of anthers responding. However, orientation of the anthers in the 'up' position with one lobe in contact with