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The effect of anther orientation on microspore-derived plant production in barley (Hordeum vulgareL.)

โœ Scribed by W. Powell; E. M. Borrino; V. Goodall

Publisher
Springer
Year
1988
Tongue
English
Weight
319 KB
Volume
38
Category
Article
ISSN
0014-2336

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โœฆ Synopsis

The effect of anther orientation on culture response has been examined in a range of barley cultivars of agronomic importance. Anther orientation did not significantly affect the percentage of anthers responding. However, orientation of the anthers in the 'up' position with one lobe in contact with the medium significantly increased the number of green plants produced when compared to anthers cultured in the 'flat' position. Green plantlet production was observed directly from embryoids i.e. without an intermediate callus phase. Cytological examination of the regenerants indicated that the majority of the plantlets possessed the normal barley karyotype. The implications of these results for Barley breeding are discussed.

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