✦ LIBER ✦

Effect of anther orientation on microspore-callus production in barley (Hordeum vulgareL.)

✍ Scribed by P. R. Mark Shannon; Anne E. Nicholson; Jim M. Dunwell; D. Roy Davies

Publisher: Springer
Year: 1985
Tongue: English
Weight: 897 KB
Volume: 4
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00040201

No coin nor oath required. For personal study only.

✦ Synopsis

Barley anthers from cold pretreated spikes produced no or few calluses when plated with both loculi in contact with the medium ('flat'). When anthers were plated with only one loculus in contact with the medium ('up'), a high proportion of the anthers produced calluses. The top loculus of the 'up' anthers was most productive. 'Flat' anthers, when compared with 'up' anthers, were not only slower to produce multicellular pollen grains (MCPs) and microcalluses, but also produced fewer of them and ceased production earlier. The MCPs and microcalluses in 'flat' anthers grew more slowly and few developed beyond the 30 cell stage. These results establish the importance of anther orientation for barley anther culture.

📜 SIMILAR VOLUMES

The effect of anther orientation on micr

The effect of anther orientation on microspore-derived plant production in barley (Hordeum vulgareL.)

✍ W. Powell; E. M. Borrino; V. Goodall 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 319 KB

The effect of anther orientation on culture response has been examined in a range of barley cultivars of agronomic importance. Anther orientation did not significantly affect the percentage of anthers responding. However, orientation of the anthers in the 'up' position with one lobe in contact with

The influence of genotype and temperatur

The influence of genotype and temperature pre-treatment on anther culture response in barley (Hordeum vulgareL.)

✍ W. Powell 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 582 KB

A~tract. The influence of temperature stress pre-treatment on anther culture response has been examined in eight commercially desirable barley cultivars. Spikes were pre-treated in darkness at 4°C for periods of 0, 7, 14, 21 and 28 days. Overall, the optimum pre-treatment period was 21 days, althoug

Effects of incubation temperature on mic

Effects of incubation temperature on microspore callus production and plant regeneration in wheat anther cultures

✍ Bin Huang 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 194 KB

The effect of cefotaxime on the growth a

The effect of cefotaxime on the growth and regeneration of callus from four varieties of barley (Hordeum vulgareL.)

✍ R. J. Mathias; C. Mukasa 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 342 KB

The effect of anther orientation on the

The effect of anther orientation on the production of microspore-derived embryoids and plants ofHordeum vulgarecv. Sabarlis

✍ C. P. Hunter 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 434 KB

The effect of different promoter-sequenc

The effect of different promoter-sequences on transient expression ofgusreporter gene in cultured barley (Hordeum vulgareL.) cells

✍ Ravindra N. Chibbar; Kutty K. Kartha; Raju S. S. Datla; Nick Leung; Karen Caswel 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 450 KB

The cauliflower mosaic virus 35S (35S) and the enhanced 35S (E35S) promoters fused with maize alcohol dehydrogenase (Adh1) intron1 or maize shrunken locus (sh1) intronl along with maize Adh1 and rice actin (Act1) promoters fused to their respective first introns were tested for transient expression