✦ LIBER ✦

Barley anther culture: Pretreatment on mannitol stimulates production of microspore-derived embryos

✍ Scribed by Sarah L. Roberts-Oehlschlager; James M. Dunwell

Publisher: Springer
Year: 1990
Tongue: English
Weight: 875 KB
Volume: 20
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00041887

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✦ Synopsis

A period of four days preincubation at 25 °C on a medium containing mannitol was found to be superior to those pretreatments requiring incubation at 4 °C. In addition, the yield of green plants was improved by orienting anthers 'flat' on the medium during mannitol preincubation, and reducing the number of anthers cultured per dish.

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