Total Stepwise Solid-Phase Synthesis of Oligonucleotide-(3‘→ N )-Peptide Conjugates
✍ Scribed by Stetsenko, Dmitry A.; Malakhov, Andrey D.; Gait, Michael J.
- Book ID
- 127016986
- Publisher
- American Chemical Society
- Year
- 2002
- Tongue
- English
- Weight
- 59 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1523-7060
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A straightforward stepwise method for the preparation of peptide-oligonucleotide phosphorothioate conjugates, was developed, based on the highly efficient Fmoc peptide solid phase synthesis, followed by oligonucleotide phosphorothiate chain assembly. The three conjugates synthesized contained 15-or
Three peptide-oligonucleotide phosphorothioate hybrids were synthesized using a new approach for stepwise solidphase synthesis of conjugates. This method utilizes commercially available N a -Fmoc amino acids for peptide synthesis and a new solid support. Three specific modifications of the solid-pha