A new solid-phase synthesis of oligonucleotides 3′-conjugated with peptides
✍ Scribed by L De Napoli; A Messere; D Montesarchio; G Piccialli; E Benedetti; E Bucci; F Rossi
- Book ID
- 108480698
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 179 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0968-0896
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A straightforward stepwise method for the preparation of peptide-oligonucleotide phosphorothioate conjugates, was developed, based on the highly efficient Fmoc peptide solid phase synthesis, followed by oligonucleotide phosphorothiate chain assembly. The three conjugates synthesized contained 15-or
Three peptide-oligonucleotide phosphorothioate hybrids were synthesized using a new approach for stepwise solidphase synthesis of conjugates. This method utilizes commercially available N a -Fmoc amino acids for peptide synthesis and a new solid support. Three specific modifications of the solid-pha