✦ LIBER ✦

Tissue porphyrin pattern determination by high-speed high-performance liquid chromatography

✍ Scribed by Sean W. Kennedy; Donald C. Wigfield; Glen A. Fox

Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 581 KB
Volume: 157
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(86)90187-9

No coin nor oath required. For personal study only.

✦ Synopsis

A rapid and sensitive method for the determination of porphyrin carboxylic acids in liver, kidney, and spleen by high-speed high-performance liquid chromatography is described. Porphyrins were extracted with recoveries &98%, concentrated on disposable octadecylsilyl cartridges, and analyzed with a liquid chromatograph equipped with a 3 pm X 3 cm octadecylsilyl column and a fluorescence detector. Separation of di-, tetra-, penta,-hexa-, hepta-, and octacarboxylic acids was achieved within 5 min. The detection limits for uro, copro, and protoporphyrin were 20, 10, and 20 fmol, respectively.

📜 SIMILAR VOLUMES

Free malonaldehyde determination in tiss

Free malonaldehyde determination in tissues by high-performance liquid chromatography

✍ A.Saari Csallany; Ming Der Guan; John D. Manwaring; Paul B. Addis 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 561 KB

A high-performance liquid chromatographic method was developed for the quantification of free malonaldehyde (MA) in tissues. HPLC separation was performed using a TSK GlOOO PW column (7.5mm i.d. X 30 cm) with a mobile phase of 0.1 M Na,P04 buffer, pH 8.0, at a Bow rate of 0.6 ml/min. The eluant was

Determination of nucleotide pools in pla

Determination of nucleotide pools in plant tissue by high-performance liquid chromatography

✍ Reiner Meyer; Karl G. Wagner 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 647 KB

Determination of lysinoalanine by high p

Determination of lysinoalanine by high performance liquid chromatography

✍ Moret, Sabrina ;Cherubin, Susi ;Rodriguez-Estrada, Maria Teresa ;Lercker, Giovan 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 412 KB

## Abstract This work suggests an HPLC method for qualitative and quantitative determination of __N__^ε^(2‐amino‐2‐carboxyethyl)‐L‐lysine (LAL). LAL was released from total hydrolysates of various proteins of animal origin and derivatized with dansyl chloride. The performance of two different colum

Determination of cystamine by high-perfo

Determination of cystamine by high-performance liquid chromatography

✍ Shuji Ida; Yoshiko Tanaka; Seitaro Ohkuma; Kinya Kuriyama 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 412 KB

A highly sensitive and specific assay method for cystamine using high-performance liquid chromatography has been developed. The method is based on postcolumn derivatization of cystamine with o-phtalaldehyde in the presence of 2-mercaptoethanol and sodium hypochlorite. The separation of cystamine was

Fluorometric determination of haloperido

Fluorometric determination of haloperidol by high performance liquid chromatography

✍ Tsuneyoshi, T. 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 226 KB

Chromatograms of 2% test mixture with semimicrobore system. (a) Low dispersion flow path (b) Dead volume 1/16 in.-1/16 in. coupling in Components: 1 rhamnose; 2 fructose; 3 glucose; 4 sucrose; 5 lactose. 0 10 m i n 0 mi " 1 0 ## 4 Conclusion Semi-microbore columns can be used with modified commerc

Determination of urinary oxalate by high

Determination of urinary oxalate by high-performance liquid chromatography

✍ H. Hughes; L. Hagen; R.A.L. Sutton 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 222 KB

A procedure for the estimation of oxalic acid in urine based upon its quantitative reaction with 1,2-diaminobenzene and subsequent estimation of the quinoxaline by reverse-phase highperformance liquid chromatography has been developed. The method has given good reproducibility; recovery of added oxa