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Tissue culture response ofBetagermplasm: callus induction and plant regeneration

✍ Scribed by J. Abe; H. Nakashima; K. Mitsui; T. Mikami; Y. Shimamoto

Publisher
Springer
Year
1991
Tongue
English
Weight
610 KB
Volume
27
Category
Article
ISSN
0167-6857

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✦ Synopsis

Callus cultures of 18 sugarbeet (Beta vulgaris) lines, two accessions of B. maritima and a B. macrocarpa accession were initiated from aseptically germinated seeds. Plant regeneration through organogenesis was obtained either on MS or B5 medium containing various concentrations and combinations of naphthaleneacetic acid (NAA), 6-benzylaminopurine (BAP), 2,3,5-triiodobenzoic acid (TIBA) and abscisic acid (ABA). Genotypes differed in their abilities of callus formation and regeneration: seven out of 18 sugarbeet lines, and an accession of B. maritima were capable of regenerating plantlets. Our data also indicated that 2/zM TIBA promoted morphogenesis from callus culture in the presence of 5/zM BAP.

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