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Callus induction and plant regeneration inVetiveria zizanioides

✍ Scribed by Marco Mucciarelli; Marisa Gallino; Silvano Scannerini; Massimo Maffei

Publisher
Springer
Year
1993
Tongue
English
Weight
915 KB
Volume
35
Category
Article
ISSN
0167-6857

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✦ Synopsis

Callus induction was obtained from basal parts of Vetiveria zizanioides Stapf. leaves cultured on Murashige and Skoog (MS) medium supplemented with 9.0 txM 2,4-dichlorophenoxyacetic acid (2,4-D), 5.7 txM indoleacetic acid (IAA) and 4.6 IxM kinetin. Calli were maintained on MS medium with the addition of 0.9 ~M 2,4-D and 2.3 IxM kinetin. Shoot formation was obtained from fast growing 14-day-old callus on the same basal medium supplemented with 0.9 txM 2,4-D and 9.3 p~M kinetin. Embryo-like structures were observed. When transferred to basal medium, shoots readily developed roots. Fully developed regenerated plants were then successfully established in soil.

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