✦ LIBER ✦

Tissue culture ofHypericum brasilienseChoisy: Shoot multiplication and callus induction

✍ Scribed by M. A. Cardoso; D. E. Oliveira

Publisher: Springer
Year: 1996
Tongue: English
Weight: 341 KB
Volume: 44
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00048184

No coin nor oath required. For personal study only.

✦ Synopsis

Hypericum brasiliense, anon-domesticated plant has been shown to have useful medicinal properties. This plant has not been cultivated so a protocol for mass propagation based on selection of superior clones was developed and a protocol established for the culture of callus cells that could be used for in vitro metabolite production. A micropropagation method based on amplification of nodal buds was developed, by selection, from ten seedling clones that were examined for growth rate, multiplication rate and rooting. The effect of various basal media, growth regulator types and concentrations were examined for optimal callus induction. Optimal callus induction occured on either Murashige and Skoog's or Gamborg's media supplemented with 1 to 2 mg 1-1 of 2,4-dichlorophenoxyacetic acid.

📜 SIMILAR VOLUMES

Tissue culture response ofBetagermplasm:

Tissue culture response ofBetagermplasm: callus induction and plant regeneration

✍ J. Abe; H. Nakashima; K. Mitsui; T. Mikami; Y. Shimamoto 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 610 KB

Callus cultures of 18 sugarbeet (Beta vulgaris) lines, two accessions of B. maritima and a B. macrocarpa accession were initiated from aseptically germinated seeds. Plant regeneration through organogenesis was obtained either on MS or B5 medium containing various concentrations and combinations of n

Triterpenoid saponin production in callu

Triterpenoid saponin production in callus and multiple shoot cultures of Gypsophila SPP.

✍ Dominique Pauthe-Dayde; Mohamed Rochd; Max Henry 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 410 KB

Callus and shoot formation in organ and

Callus and shoot formation in organ and tissue cultures ofHedera helixL., English ivy

✍ Marianne S. Banks; Mary R. Christensen; Wesley P. Hackett 📂 Article 📅 1979 🏛 Springer-Verlag 🌐 English ⚖ 220 KB

Whole embryos and organs excised from ungerminated embryos and from seedlings of H. helix were cultured to assess their capacity to form callus and regenerate organs. There is a high potential for shoot formation in the explanted cotyledons and in the explanted hypocotyl of the ungerminated embryo.