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Threonine dehydratase of streptomycin-dependent Escherichia coli K-12

✍ Scribed by I.D. Desai; W.J. Polglase

Book ID
115742238
Publisher
Elsevier Science
Year
1966
Weight
149 KB
Volume
114
Category
Article
ISSN
0005-2787

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Molecular cloning and expression of the
Molecular cloning and expression of the biodegradative threonine dehydratase gene (tdc) of Escherichia coli K12
✍ Goss, Thomas J. ;Datta, Prasanta πŸ“‚ Article πŸ“… 1985 πŸ› Springer 🌐 English βš– 944 KB

The biodegradative threonine dehydratase gene (tdc) of Escherichia coli was cloned by isolating a dehydratase negative mutant after Tn5 mutagenesis, cloning the tdc::Tn5 DNA into pBR322 and then replacing the Tn5 element on the plasmid in vivo. Subcloning and nucleotide sequence data revealed two di