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Cell preparation for the assay of threonine dehydratase in Escherichia coli

โœ Scribed by I.D. Desai

Book ID
115731776
Publisher
Elsevier Science
Year
1968
Weight
139 KB
Volume
167
Category
Article
ISSN
0005-2744

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The biodegradative threonine dehydratase gene (tdc) of Escherichia coli was cloned by isolating a dehydratase negative mutant after Tn5 mutagenesis, cloning the tdc::Tn5 DNA into pBR322 and then replacing the Tn5 element on the plasmid in vivo. Subcloning and nucleotide sequence data revealed two di