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Covalent Structure of Biodegradative Threonine Dehydratase of Escherichia coli: Homology with Other Dehydratases

โœ Scribed by Prasanta Datta, Thomas J. Goss, John R. Omnaas and Rajkumar V. Patil

Book ID
123633915
Publisher
National Academy of Sciences
Year
1987
Tongue
English
Weight
848 KB
Volume
84
Category
Article
ISSN
0027-8424

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Molecular cloning and expression of the
Molecular cloning and expression of the biodegradative threonine dehydratase gene (tdc) of Escherichia coli K12
โœ Goss, Thomas J. ;Datta, Prasanta ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Springer ๐ŸŒ English โš– 944 KB

The biodegradative threonine dehydratase gene (tdc) of Escherichia coli was cloned by isolating a dehydratase negative mutant after Tn5 mutagenesis, cloning the tdc::Tn5 DNA into pBR322 and then replacing the Tn5 element on the plasmid in vivo. Subcloning and nucleotide sequence data revealed two di