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Increased expression of biodegradative threonine dehydratase of Escherichia coli by DNA gyrase inhibitors

โœ Scribed by Venil N. Sumantran; Anthony J. Tranguch; Prasanta Datta

Book ID
109314997
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
339 KB
Volume
65
Category
Article
ISSN
0378-1097

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Molecular cloning and expression of the
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โœ Goss, Thomas J. ;Datta, Prasanta ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Springer ๐ŸŒ English โš– 944 KB

The biodegradative threonine dehydratase gene (tdc) of Escherichia coli was cloned by isolating a dehydratase negative mutant after Tn5 mutagenesis, cloning the tdc::Tn5 DNA into pBR322 and then replacing the Tn5 element on the plasmid in vivo. Subcloning and nucleotide sequence data revealed two di