Genetic characterization of streptomycin-resistant and-dependent mutants of Escherichia coli K12
✍ Scribed by Sánchez-Anzaldo, F. J. ;Bastarrachea, Fernando
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 1003 KB
- Volume
- 130
- Category
- Article
- ISSN
- 0026-8925
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Mutants resistant to (Str-R) or dependent on streptomycin (Str-D) were isolated from several streptomycin independent (Str-I) strains of Escherichia coli. From 90 of these mutants ribosomes were isolated and the ribosomal proteins analyzed by two-dimensional polyacrylamide gel electrophoresis. The r
The capablity of ribosomes of four types of streptomycin-resistant mutants (A1, A2, A40 and A60) for non-enzymatic (EF-G--GTP-independent) translocation was tested. It was found that an A40 type mutation (amino acid replacement in position 87 of the protein S12 polypeptide chain) leads to activation