𝔖 Bobbio Scriptorium
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Three-Phase Partitioning of Crude Protein Extracts

✍ Scribed by Jenó Szamos; Éva Kiss


Book ID
102575921
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
159 KB
Volume
170
Category
Article
ISSN
0021-9797

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Three-phase partitioning is an elegant way to separate proteins directly from even the large volumes of crude suspensions. It was found that interfacing it with a metal-affinity-based step makes the technique highly selective. As "proof of the concept," soybean trypsin inhibitor was purified 13-fold