Three-Phase Affinity Partitioning of Pro
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Ipsita Roy; Munishwar N. Gupta
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Article
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2002
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Elsevier Science
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English
⚖ 93 KB
Three-phase partitioning is an elegant way to separate proteins directly from even the large volumes of crude suspensions. It was found that interfacing it with a metal-affinity-based step makes the technique highly selective. As "proof of the concept," soybean trypsin inhibitor was purified 13-fold