𝔖 Bobbio Scriptorium
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Three-Phase Affinity Partitioning of Proteins

✍ Scribed by Ipsita Roy; Munishwar N. Gupta

Book ID
102560259
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
93 KB
Volume
300
Category
Article
ISSN
0003-2697

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✦ Synopsis

Three-phase partitioning is an elegant way to separate proteins directly from even the large volumes of crude suspensions. It was found that interfacing it with a metal-affinity-based step makes the technique highly selective. As "proof of the concept," soybean trypsin inhibitor was purified 13-fold with 72% recovery. As recombinant proteins (via their polyhistidine tags) and many other naturally occurring proteins are often purified by immobilized metal ion affinity chromatography, the technique described here should prove valuable in purifying biotechnologically important proteins at a large scale.

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