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Three Phase Partitioning: Concentration and Purification of Proteins

โœ Scribed by Clive Dennison; Rex Lovrien


Book ID
115645215
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
228 KB
Volume
11
Category
Article
ISSN
1046-5928

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๐Ÿ“œ SIMILAR VOLUMES


Three-Phase Affinity Partitioning of Pro
โœ Ipsita Roy; Munishwar N. Gupta ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 93 KB

Three-phase partitioning is an elegant way to separate proteins directly from even the large volumes of crude suspensions. It was found that interfacing it with a metal-affinity-based step makes the technique highly selective. As "proof of the concept," soybean trypsin inhibitor was purified 13-fold