โ Scribed by J. R. Fooks; G. L. Mattok
No coin nor oath required. For personal study only.
Chromatographic systems are described which are suitable for the examination of procaine penicillin G preparations which may contain, in addition to procaine and penicillin G, the degradation products paminobenzoic acid, ethyl p-aminobenzoate, benzylpenicilloic acid, and benzylpenillic acid. Further decomposition of penicillin G occurs when alcoholic solutions are used for spotting the chromtograms, the corresponding alkyl a-D-penicilloate being formed, whereas only penicillin G wa? detected when freshly prepared aqueous solutions were used. However, in aged aqueous solutions the rate of decomposition of penicllin G increased markedly and two new spots, due to benzylpenicilloic acid and benzylpenillic acid, were detected on the chromatogram.
๐ SIMILAR VOLUMES
Thin-layer chromatographic systems and spray reagents are described for the rapid differentiation of 10 penicillins. Comparative behavior of 18 other antibiotics under these conditions is presented together with other supporting data.
Quantitative studies were made o n the effects of various additives such as procaine hydrochloride, dextrose, and citrate in reducing penicillin G procaine solubility in water. Aqueous vehicles were prepared i n which the solubility was lowered to a minimum of 0.036 mg./cc. at room temperature. ONG