A replica plating method for isolating it amoebal mutants of Physarum polycephalum has been devised. Temperature-sensitive mutations occur at a frequency after nitrosoguanidine mutagenesis of 10(-3) per survivor, are stable but are not usually expressed in the plasmodia formed from these amoebae in
The Uptake and Metabolism of Uridine by the Slime Mould Physarum polycephalum
โ Scribed by Barbara BIRCH; Geoffrey TURNOCK
- Book ID
- 115115645
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 613 KB
- Volume
- 69
- Category
- Article
- ISSN
- 1432-1327
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๐ SIMILAR VOLUMES
Methods are described for the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum. Nineteen temperature-sensitive strains were found by screening plasmodia derived from mutagenised amoebae and the properties of these are described. A scheme is outlined for the
Microplasmodia of P. polycephalum were grown either in batch culture, in both complex and defined media to give a 3-4 fold variation in growth rate, or in a chemostate. The protein/DNA ratio of batch cultures was almost invariant, whilst the RNA/DNA ratio increased as a non-linear function of growth