The Preparation and Preliminary Characterisation of Chromatin from the Slime Mould Physarum polycephalum
โ Scribed by Brigitte M. JOCKUSCH; Ian O. WALKER
- Book ID
- 115114377
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- English
- Weight
- 843 KB
- Volume
- 48
- Category
- Article
- ISSN
- 1432-1327
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A replica plating method for isolating it amoebal mutants of Physarum polycephalum has been devised. Temperature-sensitive mutations occur at a frequency after nitrosoguanidine mutagenesis of 10(-3) per survivor, are stable but are not usually expressed in the plasmodia formed from these amoebae in
Methods are described for the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum. Nineteen temperature-sensitive strains were found by screening plasmodia derived from mutagenised amoebae and the properties of these are described. A scheme is outlined for the
Microplasmodia of P. polycephalum were grown either in batch culture, in both complex and defined media to give a 3-4 fold variation in growth rate, or in a chemostate. The protein/DNA ratio of batch cultures was almost invariant, whilst the RNA/DNA ratio increased as a non-linear function of growth
From plasmodia of the slime mould Physarum polycephalum a yellow pigment physarochrome A (1) has been obtained. Its structure has been established as all-trans N-[ll-~2-acetylamino-3-hydroxyphenyl)-2,4,6,8,1O-undecapentaenoyl]-S-glutami(l) ij;, the basis of spectroscopic evidence and hydrogenation t