A replica plating method for isolating it amoebal mutants of Physarum polycephalum has been devised. Temperature-sensitive mutations occur at a frequency after nitrosoguanidine mutagenesis of 10(-3) per survivor, are stable but are not usually expressed in the plasmodia formed from these amoebae in
Temperature-sensitive mutants of the slime mould Physarum polycephalum
โ Scribed by Gingold, Elliot C. ;Grant, William D. ;Wheals, Alan E. ;Wren, Marian
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 482 KB
- Volume
- 149
- Category
- Article
- ISSN
- 0026-8925
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โฆ Synopsis
Methods are described for the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum. Nineteen temperature-sensitive strains were found by screening plasmodia derived from mutagenised amoebae and the properties of these are described. A scheme is outlined for the detection of specific mitotic cycle lesions amongst temperature-sensitive strains, and the properties of a presumptive mitotic cycle mutant are described.
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Microplasmodia of P. polycephalum were grown either in batch culture, in both complex and defined media to give a 3-4 fold variation in growth rate, or in a chemostate. The protein/DNA ratio of batch cultures was almost invariant, whilst the RNA/DNA ratio increased as a non-linear function of growth
From plasmodia of the slime mould Physarum polycephalum a yellow pigment physarochrome A (1) has been obtained. Its structure has been established as all-trans N-[ll-~2-acetylamino-3-hydroxyphenyl)-2,4,6,8,1O-undecapentaenoyl]-S-glutami(l) ij;, the basis of spectroscopic evidence and hydrogenation t
The haploid myxamoebae of Physarum polycephalum reversibly differentiate to form dormant microcysts under conditions of starvation. The thin-walled cysts can be selective recovered from a cell suspension which has been treated with the surfactant Triton X-100 to lyse amoeboid forms. Excystment, whic
## Abstract The relation between intracellular pH and the mitotic cycle of __Physarum polycephalum__ was studied by two independent techniques. Both techniques revealed a long term cycling of intracellular pH which has the same period as the mitotic cycle. Qualitative detection of the changes in in