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Temperature-sensitive mutants of the slime mould Physarum polycephalum

โœ Scribed by Gingold, Elliot C. ;Grant, William D. ;Wheals, Alan E. ;Wren, Marian


Publisher
Springer
Year
1976
Tongue
English
Weight
482 KB
Volume
149
Category
Article
ISSN
0026-8925

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โœฆ Synopsis


Methods are described for the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum. Nineteen temperature-sensitive strains were found by screening plasmodia derived from mutagenised amoebae and the properties of these are described. A scheme is outlined for the detection of specific mitotic cycle lesions amongst temperature-sensitive strains, and the properties of a presumptive mitotic cycle mutant are described.


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## Abstract The relation between intracellular pH and the mitotic cycle of __Physarum polycephalum__ was studied by two independent techniques. Both techniques revealed a long term cycling of intracellular pH which has the same period as the mitotic cycle. Qualitative detection of the changes in in