A replica plating method for isolating it amoebal mutants of Physarum polycephalum has been devised. Temperature-sensitive mutations occur at a frequency after nitrosoguanidine mutagenesis of 10(-3) per survivor, are stable but are not usually expressed in the plasmodia formed from these amoebae in
Radiosensitivity of the slime mould Physarum polycephalum to gamma irradiation
โ Scribed by A.P. Dmitriev; N.I. Guscha; D.M. Grodzinsky
- Book ID
- 118383364
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 243 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0098-8472
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๐ SIMILAR VOLUMES
Methods are described for the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum. Nineteen temperature-sensitive strains were found by screening plasmodia derived from mutagenised amoebae and the properties of these are described. A scheme is outlined for the
Microplasmodia of P. polycephalum were grown either in batch culture, in both complex and defined media to give a 3-4 fold variation in growth rate, or in a chemostate. The protein/DNA ratio of batch cultures was almost invariant, whilst the RNA/DNA ratio increased as a non-linear function of growth
## Abstract The relation between intracellular pH and the mitotic cycle of __Physarum polycephalum__ was studied by two independent techniques. Both techniques revealed a long term cycling of intracellular pH which has the same period as the mitotic cycle. Qualitative detection of the changes in in