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The structure of the O-specific polysaccharide from Escherichia coli O113 lipopolysaccharide

✍ Scribed by Haralambos Parolis; Lesley A.S. Parolis

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
330 KB
Volume
267
Category
Article
ISSN
0008-6215

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✦ Synopsis

The O-specific polysaccharide from Escherichia coli Ol13 lipopolysaccharide was separated from the core and lipid A by mild acid hydrolysis and purified by GPC. Methylation analysis and ]H and 13C NMR spectroscopic studies of the O-deacetylated polysaccharide allowed the determination of the structure of the pentasaccharide repeating unit of the polysaccharide which can be written as

πŸ“œ SIMILAR VOLUMES

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The polysaccharide moiety of the 022-antigen (lipopolysaccharide, LPS) consists of 2-acetamido-2deoxy-D-galactose, D-glucuronic acid, D-glucose, and D-galactose in the molar ratios 2: 1: 1: 1. Methylation analysis as well as 1D and 2D NMR spectroscopy showed that the 022 polysaccharide has the prima