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Structure of the O-specific side chain of the lipopolysaccharide from Escherichia coli O126

✍ Scribed by Tapes Bhattacharyya; Sumanta Basu

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 578 KB
Volume: 254
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(94)84255-8

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✦ Synopsis

The polysaccharide isolated from Escherichia coli 0126 lipopolysaccharide contains o-galactose, o-mannose, L-fucose, and 2-acetamido-2-deoxy-o-glucose in the molar ratios 1: 1: 1: 1. The structure of the O-antigen of the lipopolysaccharide from E. coli 0126 was established by compositional analysis, partial degradation, methylation analysis, and nuclear magnetic resonance spectroscopy. These studies revealed that the O-antigen is branched and built up of tetrasaccharide repeating units having the following structure: + 2)~B-u-Manp-(I + 3l+o-Galp-(I + 31-B-D-GlcpNAc-(1 + 2 t 1 p-L-Fucp

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