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Structure of the O-specific side chain of the Escherichia coli O128 lipopolysaccharide

โœ Scribed by Prabal Sengupta; Tapes Bhattacharyya; Alexander S. Shashkov; Helga Kochanowski; Sumanta Basu

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
392 KB
Volume
277
Category
Article
ISSN
0008-6215

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โœฆ Synopsis

The O-specific polysaccharide isolated from Escherichia coli O128 lipopolysaccharide contains D-galactose, L-fucose, and 2-acetamido-2-deoxy-D-galactose in the molar ratios 2:1:2. The primary structure of the O-specific polysaccharide from E. coli was established by compositional analysis, methylation analysis, together with 1H and 13C NMR spectroscopy including two-dimensional shift-correlated and one-dimensional NOE spectroscopy. The polysaccharide moiety was found to consist of a tetrasaccharide backbone containing D-galactose and 2-acetamido-2-deoxy-D-galactose, with L-fucose as a side chain in a branched pentasaccharide repeating unit having the following structure: [formula: see text]

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