The structure of the O-specific polysaccharide chain of the lipopolysaccharide of Salmonella arizonae O61

✍ Scribed by Evgeny V. Vinogradov; Alexander S. Shashkov; Yuriy A. Knirel; Nikolay K. Kochetkov; Janusz Dabrowski; Horst Grosskurth; Evgeny S. Stanislavsky; Elena V. Kholodkova

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 616 KB
• Volume: 231
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(92)84002-a

✦ Synopsis

The O-specific polysaccharide was obtained by mild degradation of the Salmonella arizonae O61 lipopolysaccharide with acid. It contained 2-acetamido-2-deoxy-D-glucose, 2-acetamidino-2,6-dideoxy-L-galactose (FucAm), and 7-acetamido-3,5,7,9-tetradeoxy-5-[(R)-3-hydroxybutyramido]-D- glycero-L-galacto-nonulosonic acid (Sug). On the basis of partial acid hydrolysis with 0.1 M HCl, solvolysis with anhydrous HF in methanol, and 1H- and 13C-NMR analysis (including 1H/13C inversely correlated spectroscopy for localisation of N-acyl substituents), it was concluded that the O-specific polysaccharide had the following structure. ----3)-alpha-L-FucAm-(1----3)-alpha-D-GlcNAc-(1----8)-beta-Sug+ ++-(2---- The O-antigen of S. arizonae O61 is structurally related to that of Pseudomonas aeruginosa O12, thus explaining the known serological cross-reactivity between these micro-organisms.