The structure of Citrobacter O23-specific polysaccharide has been shown by sugar and methylation analyses of the native and chemically degraded polysaccharide and by 1H- and 13C-n.m.r. spectroscopy to consist of the tetrasaccharide repeating-units: ----4)-alpha-D-Man-(1----2)-alpha-D-Man-(1----2)-be
Structure of the O-specific polysaccharide chain of the lipopolysaccharide of Enterobacter agglomerans
✍ Scribed by Yannis Karamanos; Ossarath Kol; Jean-Michel Wieruszeski; Gérard Strecker; Bernard Fournet; René Zalisz
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 514 KB
- Volume
- 231
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The O-specific polysaccharide isolated from the lipopolysaccharide of Enterobacter agglomerans was found, by methylation analysis, periodate oxidation, oxidative deamination, and NMR spectroscopy, to
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NMR 0-Specific polysaccharide chains of lipopolysaccharides of a number of serotypes of an enterobacterium, Citrobacter freundii, have been structurally elucidated [l-3]. We now report the structure of a new 0-specific polysaccharide isolated from C. jkuzdii 028,lc.
The O-specific polysaccharide was obtained by mild degradation of the Salmonella arizonae O61 lipopolysaccharide with acid. It contained 2-acetamido-2-deoxy-D-glucose, 2-acetamidino-2,6-dideoxy-L-galactose (FucAm), and 7-acetamido-3,5,7,9-tetradeoxy-5-[(R)-3-hydroxybutyramido]-D- glycero-L-galacto-n
The structure of the O-specific polysaccharide of Klebsiella pneumoniae O1K2 lipopolysaccharide was investigated by use of methylation, periodate oxidation, partial hydrolysis, and 1H- and 13C-n.m.r. spectroscopy. It was shown to consist of a linear chain composed of two disaccharide repeating units
Lipopolysaccharide (LPS) was isolated from Yersinia enterocolitica serovars O : 10 and O : 10 KL and the structural pattern of O-specific sugar chains elucidated. The rhamnan and L-xylulose (L-threo-pent-2-ulose) as constituents of the O-specific polysaccharide were obtained by autohydrolysis of the