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Structure of the O-specific polysaccharide of Salmonella arizonae O45

โœ Scribed by Alexander S. Shashkov; Evgeny V. Vinogradov; Yuriy A. Knirel; Nikolay E. Nifant'ev; Nikolay K. Kochetkov; Janusz Dabrowski; Elena V. Kholodkova; Evgeny S. Stanislavsky

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
757 KB
Volume
241
Category
Article
ISSN
0008-6215

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โœฆ Synopsis

The O-specific polysaccharide of Salmonella arizonae 045 (Arizona 11) is acidic and has a branched hexasacharide repeating unit containing two residues of L-fucose, one residue each of o-galactose, D-ribose, o-glucuronic acid, and 2-acetamido-2-deoxy-D-glucose, and an 0-acetyl group. It was studied with the help of 'H and 13C NMR spectroscopy, including 1D selective spin-decoupling and homonuclear Hartmann-Hahn spectroscopy, 2D homonuclear and 13C-'H heteronuclear shift-correlated (COSY) and NOE (ROESY) spectroscopy, as well as by methylation analysis, and selective cleavages with anhydrous HF (or dilute HCI) and lithium in ethylenediamine to yield two different tetrasaccharide fragments.

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