The O-specific polysaccharide from Escherichia coli Ol13 lipopolysaccharide was separated from the core and lipid A by mild acid hydrolysis and purified by GPC. Methylation analysis and ]H and 13C NMR spectroscopic studies of the O-deacetylated polysaccharide allowed the determination of the structu
Structure of the O-specific polysaccharide of the O22-antigen (LPS) from Escherichia coli O22:K13
β Scribed by Monika Bartelt; Alexander S. Shashkov; Helga Kochanowski; Barbara Jann; Klaus Jann
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 551 KB
- Volume
- 254
- Category
- Article
- ISSN
- 0008-6215
No coin nor oath required. For personal study only.
β¦ Synopsis
The polysaccharide moiety of the 022-antigen (lipopolysaccharide, LPS) consists of 2-acetamido-2deoxy-D-galactose, D-glucuronic acid, D-glucose, and D-galactose in the molar ratios 2: 1: 1: 1. Methylation analysis as well as 1D and 2D NMR spectroscopy showed that the 022 polysaccharide has the primary structure * 6bD-Glcp-(1 + 4)-&o-GlcpA-(1 + 4)+mGalpNAc_(l + 3)-m-D-Galp-(1 + 3)-p-D-GalpNAc_(l ---) 3 6Ac
π SIMILAR VOLUMES
The O-specific side-chain of the lipopolysaccharide from Escherichia coli 090 has been investigated using methylation analysis, partial hydrolysis, and NMR spectroscopy as the principal methods. It is concluded that the polysaccharide is composed of tetrasaccharide repeating-units having the followi
The structure of the O-specific side chain of the E. coli O26 lipopolysaccharide has been investigated. Based on sugar and methylation analyses, and 2D NMR spectroscopy employing HMBC experiments, it is concluded that the polysaccharide is composed of trisaccharide repeating units having the followi
The structure of the O-antigen polysaccha~de of ~~cher~chia co& 04 has been investigated using n.m.r. spectroscopy, methylation analysis, and various specific degradations. It is concluded that the O-antigen is composed of pentasaccharide repeating-units having the following structure. ~3)-~-D-GlcpN
The polysaccharide moiety of the O83 antigen (lipopolysaccharide, LPS) consists of D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose, and D-glucuronic acid in the molar ratios 1:2:1:1. Methylation analysis of the polysaccharide and derived oligosaccharides as well as one- and two-dimensional 1H