The O-specific side-chain of the lipopolysaccharide from Escherichia coli 0127a : H-(0127a : 4932-53) has been investigated using 2D NMR spectroscopy, methylation analysis, and partial solvolysis with anhydrous hydrogen fluoride as the principal methods. It is concluded that the polysaccharide is co
Structural studies of the Escherichia coli O90 O-antigen polysaccharide
✍ Scribed by Sanjaya Ratnayake; Göran Widmalm; Andrej Weintraub; Ernesto C. Medina
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 499 KB
- Volume
- 263
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The O-specific side-chain of the lipopolysaccharide from Escherichia coli 090 has been investigated using methylation analysis, partial hydrolysis, and NMR spectroscopy as the principal methods. It is concluded that the polysaccharide is composed of tetrasaccharide repeating-units having the following structure. + 4)-(Y-L-Fucp-( 1+2)-P-D-Gab-( 1 + 3)-cx-D-GalpNAc-( 1 + 3)-P-D-GalpNAc-( 1 + 2/3 I OAc The polysaccharide contains approximately one mole of O-acetyl groups per repeating unit, located on the fucose residue.
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