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Structural studies of Escherichia coli O127 O-antigen polysaccharide

✍ Scribed by Göran Widmalm; Karin Leontein

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
427 KB
Volume
247
Category
Article
ISSN
0008-6215

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✦ Synopsis

The O-specific side-chain of the lipopolysaccharide from Escherichia coli 0127a : H-(0127a : 4932-53) has been investigated using 2D NMR spectroscopy, methylation analysis, and partial solvolysis with anhydrous hydrogen fluoride as the principal methods. It is concluded that the polysaccharide is composed of tetrasaccharide repeating-units having the following structure.

--) 2)a-L-Fucp-

The polysaccharide contains approximately one mole of O-ace@ groups per repeating unit distributed over several positions.

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