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Structure of the O83-specific polysaccharide of Escherichia coli O83:K24:H31

✍ Scribed by Barbara Jann; Alexander S. Shashkov; Michael Hahne; Helga Kochanowski; Klaus Jann

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 465 KB
Volume: 261
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(94)84018-0

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✦ Synopsis

The polysaccharide moiety of the O83 antigen (lipopolysaccharide, LPS) consists of D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose, and D-glucuronic acid in the molar ratios 1:2:1:1. Methylation analysis of the polysaccharide and derived oligosaccharides as well as one- and two-dimensional 1H and 13C NMR spectroscopy of the polysaccharide at pD 1 and 6 showed that the O83 polysaccharide has the primary structure-->6)-alpha-D-Glc p-(1-->4)-beta-D-Glc pA-(1-->6)-beta-D-Gal p-(1-->4)-beta-D- Gal p-(1-->4)-beta-D-Glc pNAc-(1-->.

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