The vegetative prothalli (1-3 weeks old) of Anemia were incubated for 24 h in [t4C]adenine. The RNA was phenol extracted from whole cells and the poly (A) sequences were isolated by nuclease digestion followed by poly (U)-sepharose chromatography. About 2-3% of the total radioactivity was retained o
The sporopollenin of the schizaeaceous fernAnemia phyllitidis
โ Scribed by H. Schraudolf; R. Haag
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 296 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0028-1042
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A complete cDNA encoding y-tubulin protein from Anemia phyllitidis is presented. The deduced amino acid sequence shows an average similarity of 81-86~o to known y-tubulin genes from insects, mammals and fungi. Northern blots indicate the existence of transcripts of ca. 1.9 kb which are expressed dur