The polyadenylic sequences in the ribonucleic acid of the fernAnemia phyllitidis
β Scribed by Javed Iqbal
- Publisher
- Springer-Verlag
- Year
- 1977
- Tongue
- English
- Weight
- 279 KB
- Volume
- 134
- Category
- Article
- ISSN
- 0032-0935
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β¦ Synopsis
The vegetative prothalli (1-3 weeks old) of Anemia were incubated for 24 h in [t4C]adenine. The RNA was phenol extracted from whole cells and the poly (A) sequences were isolated by nuclease digestion followed by poly (U)-sepharose chromatography. About 2-3% of the total radioactivity was retained on the column. The base composition of the nuclease resistant RNA was: C, 1.4; G, 3.6; A, 93.3; and U, 1.7. It is concluded that Anemia RNA contains poly adenylate sequences.
π SIMILAR VOLUMES
Deletions were made in the cauliflower mosaic virus polyadenylation sequence which was cloned downstream of the beta-glucuronidase gene (gus). The populations of mRNAs generated in tobacco mesophyll protoplasts by transient expression with the various constructs were analysed using a polymerase chai
## Abstract The composition and molecular size of enzymatically synthesized polyadenylic and polyuridylic acids (polyβA, polyβUThe following abbreviations will be used: ADP, UDP, etc. 5β²βdiphosphates of adenosine, etc.; polyβA, βU, etc. polynucleotides: polyadenylic acid, etc.; poly(A + U), etc. he