Isolation, characterization and sequence of a cDNA encoding γ-tubulin protein from the fernAnemia phyllitidisL. Sw.
✍ Scribed by Ulrike Fuchs; Barbara Moepps; Helmut P. Maucher; Helmut Schraudolf
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 702 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0167-4412
No coin nor oath required. For personal study only.
✦ Synopsis
A complete cDNA encoding y-tubulin protein from Anemia phyllitidis is presented. The deduced amino acid sequence shows an average similarity of 81-86~o to known y-tubulin genes from insects, mammals and fungi. Northern blots indicate the existence of transcripts of ca. 1.9 kb which are expressed during gametogenesis of A. phyllitidis. The gene described is the first cloned and entirely sequenced y-tubulin gene in any plant.
📜 SIMILAR VOLUMES
Phytolacca anti-viral protein (PAP) was purified from Phytolacca leaves and the N-terminal was sequenced. A cDNA library was made from mRNAs isolated from Phytolacca leaves and cDNA clones for PAP were identified using oligonucleotide probes derived from the N-terminal amino acid sequence. The PAP-c
The sequence of an mRNA encoding nitrite reductase (NiR, EC 1.7.7.1.) from the tree Betula pendula was determined. A cDNA library constructed from leaf poly(A)+ mRNA was screened with an oligonucleotide probe deduced from NiR sequences from spinach and maize. A 2.5 kb cDNA was isolated that hybridiz