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Isolation and characterization of a cDNA clone encoding the anti-viral protein fromPhytolacca americana

✍ Scribed by Q. Lin; Z. C. Chen; J. F. Antoniw; R. F. White

Publisher
Springer
Year
1991
Tongue
English
Weight
421 KB
Volume
17
Category
Article
ISSN
0167-4412

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✦ Synopsis

Phytolacca anti-viral protein (PAP) was purified from Phytolacca leaves and the N-terminal was sequenced. A cDNA library was made from mRNAs isolated from Phytolacca leaves and cDNA clones for PAP were identified using oligonucleotide probes derived from the N-terminal amino acid sequence. The PAP-cDNA clone was sequenced from both directions. The predicted amino acid sequence of PAP was compared with the amino acid sequences of other ribosome-inactivating proteins. The identities of these proteins to PAP ranged from 29 to 38~o, and a region was found in each with a sequence similar to the PAP sequence (AIQMVSEAARFKYI). Southern blot analysis indicates that PAP is encoded by a multi-gene family.

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