The Preparation of C 14 -Chain Labeled Choline Chloride
β Scribed by Dauben, William G.; Gee, Mildred
- Book ID
- 120217430
- Publisher
- American Chemical Society
- Year
- 1952
- Tongue
- English
- Weight
- 295 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0002-7863
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## Abstract A modification of our previously reported^2^ gas chromatographic method of preparing vinylβ^14^C~2~ chloride (^14^CβVC) has been made. The procedure using 1,2βdichloroethaneβ^14^C~2~ obtained from New England Nuclear Corporation gave good yields of ^14^CβVC as a gas mixture with helium.
## Abstract Utilizing sodium [1β^14^C]propionate as a precursor, [^14^C]FKβ506, labeled at carbon atoms 10, 16, 18, 21a, 24, and 26, was produced by fermentative biosynthesis. Extractive isolation followed by chromatographic purification provided material of purity suitable for metabolism studies.