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The neutral carotenoids of wild-type and mutant strains ofNeurospora crassa

✍ Scribed by A. Hughes Goldie; Ronald E. Subden

Publisher: Springer
Year: 1973
Tongue: English
Weight: 480 KB
Volume: 10
Category: Article
ISSN: 0006-2928
DOI: 10.1007/bf00485705

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✦ Synopsis

The neutral carotenoids of wild-type Neurospora crassa and of carotenoid mutants at four discrete genetic loci were isolated using gradient elution chromatography on deactivated alumina columns. Carotenoids were identified by absorption spectrophotometry and thin layer cochromatography with carotenoid standards. Phytoene, phytofluene, (-carotene, fl-carotene, neurosporene, torulene, lycopene, and 3,4-dehydrolycopene were isolated from wild type. Phytoene, phytofluene, (-carotene, fl-earotene, neurosporene, y-carotene, lycopene, and one unknown carotenoid, tentatively identified as 15,15'-cis-flcarotene, were isolated from a yellow mutant, ylo-1, ylo-1 also contained residual carotenoids having similar absorption spectra to, but very different chromatographic behavior from, phytofluene, ~-carotene, fl-carotene, and lycopene. Albino and colored al-1 mutants contained large amounts of phytoene and only traces of other neutral carotenoids. Albino al-2 and al-3 mutants contained only traces of neutral carotenoids.

Reagents

Petroleum ether (boiling point 30-60 C), acetone, diethyl ether, and benzene

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