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Preparation of a cell-free translation system from a wild-type strain ofNeurospora crassa

โœ Scribed by M. Devchand; F. P. Buxton; D. I. Gwynne; R. W. Davies

Publisher
Springer-Verlag
Year
1988
Tongue
English
Weight
392 KB
Volume
13
Category
Article
ISSN
0172-8083

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โœฆ Synopsis

We describe the preparation of an in vitro translation system from a wild-type strain of Neurospora crassa. The system is capable of supporting efficient and faithful translation of native and in vitro transcribed eukaryotic messages. The translation products have minimal background and can be clearly analyzed by SDS-polyacrylamide gel electrophoresis. The method of preparation of the lysate is simple, fast and reproducible. The procedure should be readily applicable to other filamentous fungi.

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