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Regulation of the neutral phosphatase inChlamydomonas reinhardi:An immunogenetic study of wild-type and mutant strains

✍ Scribed by R. Loppes; J. Braipson; R. F. Matagne; A. Sassen; L. Ledoux


Publisher
Springer
Year
1977
Tongue
English
Weight
600 KB
Volume
15
Category
Article
ISSN
0006-2928

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✦ Synopsis


In Chlamydomonas reinhardi, the activity of the neutral phosphatase considerably increases when the cells are grown in the absence of inorganic phosphate (Pi). A comparative immunological study of cells grown on media containing Pi or not indicated that the neutral phosphatase was synthesized de novo. Ten mutants lacking the neutral phosphatase and distributed among three genetic loci (PD2, PD3, PD24) were investigated for their ability to produce cross-reacting material (CRM) antigenically related to the wild enzyme. All mutants were shown to form much less CRM than the wild-type strain. It is proposed that the three genes are involved in the regulation of neutral phosphatase synthesis.


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